In Vivo Modeling of Ischemic Stroke

MCAO Stroke Behavior
Ischemic strokes account for 80% of stroke incidence. Therefore, an in-depth understanding of the pathogenesis of ischemic stroke is crucial to attacking stroke. And in vivo ischemic stroke model is an important tool to study its pathogenesis. Many methods have been established to build different in vivo ischemic stroke models. Ace Neuroscience offers comprehensive services for building in vivo models of ischemic stroke.

in vivo Modeling of Ischemic Stroke

Intraluminal Suture MCAO Model
The middle cerebral artery (MCA) and its branches are the cerebral vessels most severely affected by ischemic stroke, accounting for approximately 70% of infarcts. Therefore, occlusion of this artery most closely resembles ischemic stroke in humans. In occlusive MCA stroke models, intra-arterial suture occlusion of the MCA (MCAO) is the most common method used in stroke studies in rodents. We have the experience and professional scientists to ensure the success of the model building, in addition, depending on experimental needs, we build permanent ischemia-reperfusion and transient ischemia-reperfusion models for your study.

Craniectomy Model
The craniectomy model is able to better reproduce infarct size and neurological impairment, it has a low mortality rate, and is able to visually confirm the success of MCAO. In addition, the model allows for subsequent reperfusion of ischemic tissue. However, the craniectomy technique may result in injury to the underlying cortex or rupture of the vessel by drilling or electrocoagulation. We have successfully overcome this problem by continuous polishing and careful improvement, which can ensure the intracranial pressure and the function of the blood brain barrier.

Photothrombosis Model
The photothrombosis model is the model that requires the least surgical intervention with low mortality and its resulting lesions are highly reproducible. However, because the arteries in this model are not occluded, only little or no ischemic penumbra and local tributary measurement/reperfusion occur. We have made technical improvements to the photothrombosis model to make it more closely resemble the pathological process of ischemic stroke in humans.

Endothelin-1 Model
The application of endothelin-1 can be effective in establishing focal stroke models. It has the advantage of less invasive techniques, low mortality, and the possibility of inducing direct focal ischemia in deep and superficial brain regions. Our scientists can establish endothelin-1 stroke models in a stable manner to ensure scientific and rigorous experiments.